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KMID : 0545119940040020085
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Journal of Microbiology and Biotechnology
1994 Volume.4 No. 2 p.85 ~ p.94
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Detection and Determination of Lipase Activity
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Lee Seoung-Yong
Rhee Joon-Shick
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Abstract
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The effects of glycine on cell growth and L-omithine production were investigated in shake-flask and jar fermentor cultures of a citrulline auxotrophic mutant, Brevibacterium ketoglutamicum BK 1046. In the shakeflask culture, the optimal concentration of glycine for L-omithine production was found to be 20 g/ℓ. In the jar fermentor culture with the glycine at an initial concentration of 20 g/ℓ, L-omithine production increased by 28%, compared to that of the culture with no glycine added. 37 g/ℓ of L-omithine was produced when additional feeding of glycine (5 g/ℓ) was made. This was a significant improvement in L-omithine production compared to that (ca. 24 g/ℓ) of the corresponding batch culture conducted without glycine. According to the stoichiometric analysis with the batch fermentation results, the experimental and theoretical L-omithine yields based on the glucose consumption were 0.24 and 0.59, respectively. This indicates that the performance of L-omithine fermentation can further be improved by the supplementation of glycine and the development of a mutant strain possessing a higher growth yield.
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